Gene description for TSG101
Gene name	tumor susceptibility 101
Gene symbol	TSG101
Other names/aliases	TSG10 VPS23
Species	Homo sapiens
Experiment description of studies that identified TSG101 in Melanoma cells
1 Experiment ID 237 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Dot blot PubMed ID 21293856    Organism Homo sapiens Experiment description Tumour exosomes inhibit binding of tumour-reactive antibodies to tumour cells and reduce ADCC Authors Battke C, Ruiss R, Welsch U, Wimberger P, Lang S, Jochum S, Zeidler R Journal name Cancer Immunol Immunother Publication year 2011 Sample Melanoma cells Sample name Mel624.38 Isolation/purification methods Differential centrifugation Filtration Ultrafiltration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.17 g/mL Molecules identified in the study Protein Methods used in the study Dot blot EV-TRACK EV110080: EV-METRIC:14%, 14%, 38%
2 Experiment ID 624 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name UACC-257 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
3 Experiment ID 625 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name UACC-62 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 986 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 25261472    Organism Homo sapiens Experiment description Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. Authors Liang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK. Journal name J Biol Chem. Publication year 2014 Sample Melanoma cells Sample name SK-MEL-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140188: EV-METRIC:22%
5 Experiment ID 1181 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 28684402    Organism Homo sapiens Experiment description BRAFV600 inhibition alters the microRNA cargo in the vesicular secretome of malignant melanoma cells Authors Lunavat TR, Cheng L, Einarsdottir BO, Olofsson Bagge R, Veppil Muralidharan S, Sharples RA, Läer C, Gho YS, Hill AF, Nilsson JA, Löll J.". Journal name Proc Natl Acad Sci U S A. Publication year 2017 Sample Melanoma cells Sample name MML-1 cells Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study miRNA Methods used in the study Next generation sequencing qPCR EV-TRACK -
6 Experiment ID 1182 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 28684402    Organism Homo sapiens Experiment description BRAFV600 inhibition alters the microRNA cargo in the vesicular secretome of malignant melanoma cells Authors Lunavat TR, Cheng L, Einarsdottir BO, Olofsson Bagge R, Veppil Muralidharan S, Sharples RA, Läer C, Gho YS, Hill AF, Nilsson JA, Löll J.". Journal name Proc Natl Acad Sci U S A. Publication year 2017 Sample Melanoma cells Sample name MML-1 cells treated with Vemurafenib Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study miRNA Methods used in the study Next generation sequencing qPCR EV-TRACK -
7 Experiment ID 2966 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name A375 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Reverse phase protein array EV-TRACK -
8 Experiment ID 2967 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name UACC-903 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Reverse phase protein array EV-TRACK -
9 Experiment ID 2968 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Reverse phase protein array Immuno-EM ELISA EV-TRACK -
10 Experiment ID 2969 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM164 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Reverse phase protein array ELISA EV-TRACK -
11 Experiment ID 2974 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 - Fraction 6 Isolation/purification methods Differential centrifugation Iodixanol density gradient centrifugation Ultracentrifugation Flotation density 1.131 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 2975 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 - Fraction 7 Isolation/purification methods Differential centrifugation Iodixanol density gradient centrifugation Ultracentrifugation Flotation density 1.144 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
13 Experiment ID 2976 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 - Fraction 8 Isolation/purification methods Differential centrifugation Iodixanol density gradient centrifugation Ultracentrifugation Flotation density 1.156 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
14 Experiment ID 2977 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 - Fraction 9 Isolation/purification methods Differential centrifugation Iodixanol density gradient centrifugation Ultracentrifugation Flotation density 1.178 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
15 Experiment ID 2978 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 - Fraction 10 Isolation/purification methods Differential centrifugation Iodixanol density gradient centrifugation Ultracentrifugation Flotation density 1.193 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
16 Experiment ID 2985 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
17 Experiment ID 2986 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
18 Experiment ID 2987 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
19 Experiment ID 2988 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
20 Experiment ID 2989 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
21 Experiment ID 2990 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name WM9 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
22 Experiment ID 3000 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name MEL624 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
23 Experiment ID 3001 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 30089911    Organism Homo sapiens Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response Authors Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W. Journal name Nature Publication year 2018 Sample Melanoma cells Sample name MEL624 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Reverse phase protein array Western blotting ELISA EV-TRACK -